ON PAPAIN LIPASE
نویسندگان
چکیده
منابع مشابه
Influence of organic solvents on papain kinetics.
Papain activity was studied in water-organic solvent mixtures using the fluorogenic substrate Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans. The increase of organic solvent (MeOH, EtOH, iPrOH, TFE, MeCN, (MeO)2Et and DMF) concentration in the mixture caused a substantial decrease the initial rate of papain-catalyzed hydrolysis. Moreover, the number of papain active sites decreased with the increase of D...
متن کاملAction of papain on human serum globulins.
Studies on the degradation of animal and human serum antibody type proteins by various proteolytic enzymes have indicated that molecules of approximately one-half and one-quarter the size of the parent 160,000-molecular weight protein may be readily formed (l-5). Porter (6) has recently shown that rabbit y-globulin is degraded by papain into fragments of the above size range and has separated s...
متن کاملAnaphylaxis induced by a drug containing lysozyme and papain: influence of papain on the IgE response.
BACKGROUND This paper reports the case of an egg-allergic pediatric patient who, once desensitized to egg following a successful rush oral immunotherapy protocol, could also tolerate Lizipaina®, a drug containing lysozyme (LYS) and papain, which had previously caused him a severe allergic reaction. Because the LYS amount that elicited the anaphylactic reaction (5 mg) was much lower than that to...
متن کاملCrystalline Papain.
In an earlier study (1) of the amino acid composition of papain, it was assumed that all of the sulfur could be attributed to cysteine and cystine since methionine is absent from this protein. However, because of the sulfhydryl nature of this enzyme, the content of these amino acids is of some importance; we have used the method of Schram, Moore, and Bigwood (2) for this estimation. The results...
متن کاملStability and unfolding studies on papain.
Papain (EC.3.4.22.2) is a cysteine proteinase extracted from the plant, Curicupupayu, which is used extensively in many industrial processes, due to its high stability and broad substrate range. The enzyme consists of a single polypeptide chain of 212 residues and has a molecular weight of 23,400 daltons [l]. The three dimensional structure has been determined to high resolution, and shows that...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1925
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)84949-3